Well done, thank you. As an aside on Stephen Buhner's plant based protocol, we have tailored the long covid protocol for several people with really good results and no side effects (some of the mentioned pHarma have nephro toxicity and other side effects). As well we have used the Covid treatment protocol for several who had active infection, again with excellent results, including one who was two weeks into his symptoms.
Mar 15, 2022·edited Mar 15, 2022Liked by Modern Discontent
Some notes on the stabilization part, with the caveat that my research here is still VERY half-baked. Essentially my understanding matches the overall gist but is even a bit worse than how you have phrased it.
The Proline subs were at the bottom (C-term) of HR1, per https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7829931/ - Also it was KV originally, so not an intuitively radical substitution compared to taking out some hydrophobes in the middle of HR1 and I don't get the impression that any real-life testing confirmed the effect.
"However, the two amino acid replacements (K986P, V987P), shown in Figure 5, stabilize the resulting spike protein in the prefusion state and contribute to vaccine efficiency. The mutant SARS-2-S spike protein with these proline replacements is referred to as S-2P [85,86], which is encoded in the mRNA vaccine from both Pfizer/BioNTech (BNT162b2) and Moderna (mRNA-1273)."
Placed here, the "stability" conferred is that HR1 can't fire upward into nearby membranes, facilitating fusion. But all the conformational changes before then are still possible. That includes premature (pre-binding) cleavage / break-off of the S1 unit., as well as ACE-2 adjacent (during binding) cleavage, though the latter might not be as bad as the former once you have locked HR1 in place.
Essentially it is like they locked the "Jack" of the jack in the box in place, but the lid can still fall off and fly around in the blood and do whatever (besides obviously being exposed to the blood anyway if it is expressed on endothelial membranes).
Taken as individual articles,or, taken as an organic whole, a series of continuous crucial explanations, the intent to help mankind is magnificent and munificent. Moreover, it is scientifically verifiable as well as crucially available at just the proper time.Sir, I respectfully submit, that you are and have been, a benefactor of mankind, an example to be emulated and repeated , if it please G-d.
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Well done, thank you. As an aside on Stephen Buhner's plant based protocol, we have tailored the long covid protocol for several people with really good results and no side effects (some of the mentioned pHarma have nephro toxicity and other side effects). As well we have used the Covid treatment protocol for several who had active infection, again with excellent results, including one who was two weeks into his symptoms.
Aloha y'all
Some notes on the stabilization part, with the caveat that my research here is still VERY half-baked. Essentially my understanding matches the overall gist but is even a bit worse than how you have phrased it.
The Proline subs were at the bottom (C-term) of HR1, per https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC7829931/ - Also it was KV originally, so not an intuitively radical substitution compared to taking out some hydrophobes in the middle of HR1 and I don't get the impression that any real-life testing confirmed the effect.
"However, the two amino acid replacements (K986P, V987P), shown in Figure 5, stabilize the resulting spike protein in the prefusion state and contribute to vaccine efficiency. The mutant SARS-2-S spike protein with these proline replacements is referred to as S-2P [85,86], which is encoded in the mRNA vaccine from both Pfizer/BioNTech (BNT162b2) and Moderna (mRNA-1273)."
Placed here, the "stability" conferred is that HR1 can't fire upward into nearby membranes, facilitating fusion. But all the conformational changes before then are still possible. That includes premature (pre-binding) cleavage / break-off of the S1 unit., as well as ACE-2 adjacent (during binding) cleavage, though the latter might not be as bad as the former once you have locked HR1 in place.
Essentially it is like they locked the "Jack" of the jack in the box in place, but the lid can still fall off and fly around in the blood and do whatever (besides obviously being exposed to the blood anyway if it is expressed on endothelial membranes).
Taken as individual articles,or, taken as an organic whole, a series of continuous crucial explanations, the intent to help mankind is magnificent and munificent. Moreover, it is scientifically verifiable as well as crucially available at just the proper time.Sir, I respectfully submit, that you are and have been, a benefactor of mankind, an example to be emulated and repeated , if it please G-d.